switch remained unknow

0 41
DMT  
WUHAN hydro flask uk sale , Sept. 22 (Xinhua) -- Police caught six tomb raiders who learned fengshui and used it to locate and loot tombs in the central Chinese provinces of Hubei and Hunan, authorities said Friday.

The "master tomb raider", identified only as Ye, was able to look at a site and determine the best place to dig by applying fengshui, a traditional Chinese study of geomantic omens used to find auspicious locations for buildings and cemeteries.

Ye hydro flask uk , 44, was once sentenced to 10 years in prison for theft and robbery. He learned knowledge of fengshui in prison, where one of the inmates told him raiding tombs was profitable.

After he was released from prison he purchased tools online, such as a Luoyang shovel, and organized a tomb-raiding group.

The police investigation started in May when they received information that people had raided a Chu State tomb from China's Warring States Period (475 to 221 B.C.).

The police then targeted a six-man raiding group. By September Hydro Flask Coffee 12 Oz Coffee Lava Red UK Sale , the suspects were caught by police.

According to Wuhan police, they seized and retrieved six cultural relics, including a bronze mirror with a diameter of 50 centimeters, the largest mirror ever unearthed in the city.

The police said that the tomb raiders had looted three tombs in Hubei, but did not disclose how many tombs they had raided in neighboring Hunan Hydro Flask Coffee 12 Oz Coffee Graphite Grey UK Sale , with the investigation ongoing.

Chinese tomb raiders can make good money, as wealthy people in China used to be buried with treasure such as gold, silverware and jade in the hope of enjoying an affluent afterlife.

Chinese, Australian armies conclude joint training exercise

Aftermath of 7.1-magnitude earthquake in Mexico City

Sacrifice ceremony for late Thai king held in Bangkok

Dominica PM confirms at least 15 killed in hurricane Maria attack

Highlights of 10th China Acrobatics Golden Chrysanthemum Awards

Wildlife paradise: Changtang National Nature Reserve in China's Tibet

PLA Navy frigates introduced to visitors at Port of Antwerp, Belgium

Aviation Expo China 2017 held in Beijing

SAN FRANCISCO Hydro Flask Coffee 12 Oz Coffee Flamingo Pink UK Sale , Sept. 20 (Xinhua) -- Researchers in the United States have identified a key region within a protein that governs how accurately the gene-editing tool known as CRISPR-Cas9 homes in on a target deoxyribonucleic acid (DNA) sequence.

CRISPR is short for Clustered Regularly Interspaced Short Palindromic Repeats and Cas9 stands for CRISPR Associated Protein.

The researchers at the University of California, Berkeley, and Massachusetts General Hospital said they have tweaked the region within the Cas9 protein to produce a hyper-accurate gene editor with the lowest level of off-target cutting to date. Identified as a master controller of DNA cutting, the protein domain known as REC3 is a target for re-engineering to further improve accuracy and to minimize the chance that CRISPR-Cas9 will edit DNA at the wrong place.

Co-first author Janice Chen, a graduate student in the lab of Jennifer Doudna Hydro Flask Coffee 16 Oz Coffee Blueberry UK Sale , the UC Berkeley professor of molecular and cell biology who was one of the first scientists to invent CRISPR-Cas9 technology, noted in a press release that "we have found that even minor alterations in the REC3 domain of Cas9 affect the differential between on- and off-target editing, which suggests that this domain is an obvious candidate for in-depth mutagenesis to improve targeting specificity."

Targeting specificity is a key consideration when doing gene therapy in humans.

Currently, scientists using CRISPR-Cas9 create a single-guide ribonucleic acid, or sgRNA Hydro Flask Coffee 20 Oz Coffee Cobalt Blue UK Sale , an RNA molecule that includes a chain of 20 ribonucleic acids that complements a specific 20-nucleic-acid DNA sequence they want to target, and attach it to Cas9. This guide RNA allows Cas9 to home in on the complementary DNA, bind to it and cut the double stranded helix.

However, the Cas9-sgRNA complex can bind to DNA that doesn't exactly match, leading to undesirable off-target cutting.

Doudna's lab discovered in 2015 a conformational switch of Cas9 that is activated when the RNA guide and DNA target match. They found that only when the RNA and DNA match closely does the 3D structure of Cas9 Hydro Flask Coffee 20 Oz Coffee Black UK Sale , in particular the conformation of the HNH nuclease domain, change and activate the scissors of Cas9. But the process for sensing the nucleic acids upstream of the conformational switch remained unknown.

In the current study supported by the U.S. National Institutes of Health (NIH) and the U.S. National Science Foundation (NSF), Chen and her colleagues used a technique called single-molecule Forster resonance energy transfer (FRET) to precisely measure how the various protein domains in the Cas9-sgRNA protein complex - in particular REC3, REC2 and HNH - move when the complex binds to DNA.

The researchers uncovered that the REC3 domain is responsible for sensing the accuracy of target binding, which then signals the outward rotation of the REC2 domain to open a path for the HNH nuclease domain Hydro Flask Hydration 18 Oz Standard Mouth Lemon Yellow UK Sale , activating the scissors. They then showed that by mutating parts of REC3, it is possible to change the specificity of the Cas9 protein so that the HNH nuclease is not activated unless the guide RNA and target DNA match is very close.

They were able to engineer an improved hyper-accurate Cas9, dubbed HypaCas9, that retains its on-target efficiency but is slightly better at discriminating between on- and off-target sites in human cells.

"If you mutate certain amino acid residues in REC3, you can tweak the balance between Cas9 on-target activity and improve specificity; we were able to find the sweet spot where there is sufficient activity at the intended target but also a large reduction in off-target events Hydro Flask Hydration 21 Oz Standard Mouth Cobalt Blue UK Sale ," Chen was quoted as saying in the news release from UC Berkeley.

Xi meets Singaporean PM on advancing ties

Rescue teams work to find survivors as Mexico quake t. Cheap Wholesale Jerseys   Cheap Authentic Jerseys   Cheap NFL Jerseys   Cheap Authentic NFL Jerseys   Cheap Custom NFL Jerseys   Cheap NFL Jerseys From China   Wholesale Jerseys Online   Wholesale Sports Jerseys   Wholesale Sports Jerseys   Cheap Retro Soccer Jerseys  



You have to log in before you can reply Login | Sign Up

Points Rules